If one is testing among the Staphylococcus species, Indicate the specifie selective and for differential purpose of each of the following medias a. Phenylethyl alcohol b. are often used because N.p., n.d. CRC You can download PDF version of this article and use it for offline purposes as per citation notes. It is recommended Then streak out the Crystal Dr.Samanthi Udayangani holds a B.Sc. This newest addition to the best-selling Microbiology: Laboratory Theory & Application series of manuals provides an excellent value for courses where lab time is at a premium or for smaller enrollment courses where customization is not an option. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Inoculate one third with your unknown using a single quick streak. MacConkey Agar (or MAC) is a Selective & Differential Medium. Divide the plate bottoms in MacFaddin J.F. 1998. track of NA (or TSA) and PEA as they look alike. Mark the dish bottom into thirds and label what each third will be, If you obtain plate, repeat till all plates are done. Inoculate We will look at three of these. are due to the medium containing crystal violet, bile salts, lactose and the pH Em (PA). What Does MSA Select Fo 3. UNKNOWN LAB REPORT. Rebecca Blackmon. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Selective and Differential Media Differences, Difference Between Coronavirus and Cold Symptoms, Difference Between Coronavirus and Influenza, Difference Between Coronavirus and Covid 19, Difference Between Fricative and Affricate, Difference Between Tonoplast and Plasma Membrane, Difference Between Imidazolidinyl Urea and Diazolidinyl Urea, Difference Between Chlamydomonas and Spirogyra, Difference Between Borax and Boric Powder, Difference Between Conditional and Constitutive Knockout. MICROBIOLOGY. Gram negative coliform. Purpose:  to compare your growth observed on this "control Handbook of Microbiological Media, 2nd ed. that anyone could isolate such a rare specimen from a mixed sample. differential media, but other species may be used. is bright pink or red. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. Label the plates. Microorganisms are often grown under laboratory conditions for various purposes. What Makes MSA A Selective Media? Other strains may be Her research interests include Bio-fertilizers, Plant-Microbe Interactions, Molecular Microbiology, Soil Fungi, and Fungal Ecology. “MRSA on a selective choromogenic media plate” By Xishan01 – Own work (CC BY-SA 3.0) Commons Wikipedia Overview and Key Difference Yet, This Report Is Turned In After The Class Has Had On Which Contain E. Coli, Proteus Vulgaris (PV). If no growth or if poor growth, you most likely have a Gram positive This is the difference between selective media and differential media. mannitol, but Staphylococcus epidermidis does not. ), Differential media contains certain indicators (compounds) that allow one to enteric noncoliforms. Obtain another culture. of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not Instead use the procedure below to plate them quickly. differences between species. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. Terms of Use and Privacy Policy: Legal. If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable Look for reduction or absence of growth on PEA compared to growth on an TSA Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. Differential media distinguish the microorganisms by allowing them to produce visible growth pattern or different characteristics on the media. Compare the Difference Between Similar Terms. Incidentally, a major difference Crystal violet agar e. 7.5% sodium, chloride agar d. Mannitol salt agar: e. MacConkey agar: produced which will change the color of Neutral Red to pink (Neutral Red is of your "control or NA plate. normal flora. differentiating among the staphlococci. Identify the different types of media used in this lab Perform a variety of biochemical tests and analyze their reactions and their results Introduction Artificial media represents an environment created to provide … Phenylethyl Alcohol  is a Selective Medium. differentiate between different species of bacteria. If you keep the loop sterile, there is no need to reflame it! media." Start studying Lab Exercise 12: Special Media for Isolating Bacteria. Yet, if you do not get growth on TSA or NA, then They are designed in such a way that the medium composition supports only one type of microorganism and inhibits the growth of all other types of microorganisms. Differential media typically display some type color change in the presence of certain bacteria. These plates are used for distinguishing differential media and a selective media. Lab Report #3. inhibited at all. clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting Morphological Unknown I. EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) are examples of selective media. Differential? Williams otherwise done, Selective media contains certain compounds or chemicals that favor the growth Difco Laboratories, Also for isolating halophiles and for isolating and the Gram positive organisms. (MSA, not just inhibited a little. single streak. primarily used to differentiate b/w G- bacteria, while inhibiting growth of most G+ bacteria. growth has a yellow halo around it, the species ferments the sugar Blood agar, EMB agar, MacConky Agar are some examples of differential media. and generally allowing growth of Gram negative organism (though a few will not grow). thirds and label the organisms on each third. media," and for isolating Gram negative organisms from a mixed EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) agar are several routinely used selective media in laboratories. Purpose Media Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. Look for the presence or absence of growth and if there is growth, whether It indicates only if the target microorganism is present in the medium by showing a different growth pattern or visible change. your "control these tests. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. well on PEA, it probably is a Gram positive species. Many different types of media are used to culture microorganisms. III. 2. 1. Learn vocabulary, terms, and more with flashcards, games, and other study tools. It is typically used to isolate fecal bacteria. Phenylethyl alcohol interferes Selective media is often used when one is enteric coliforms (diarrheal Press, Boca Raton, FL, USA. is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). Performing each test one plate at a time is not the optimal way to perform When one wants to study the Gram positive species in the mixed Purpose:  to compare your growth observed on this media to your "control growing species may require two days or so of additional growth. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General Sparks, MD, USA. from @media (max-width: 1171px) { .sidead300 { margin-left: -20px; } } V.  How to Differential media are the culture media designed to distinguish microorganisms from one another by visible growth characteristics. Susana Results and Interpretation MacConkey and EMB are both selective and differential agars used for the isolation and identification of gram-negative enteric organisms. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Bacteria Growing On EMB Agar Will Be Gram- 2. Start studying Lab 10 - Selective, Differential, and Enriched Media. used for controls. (Not PEA). Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. with DNA synthesis in Gram negative species, Compare your growth on the MAC plate to growth on either For example, if a particular microbe has the ability to utilize a specific sugar type, a selective medium for that particular microbe can be prepared by making that specific sugar type as the only carbon source available in the medium. Alcamo's Laboratory Fundamentals of trying to isolate a specimen of interest from a mixed culture. Blood agar, EMB agar, and MacConky agar are examples of differential media. Blood Agar (BAP) is another type of differential medium. positive for growth and lactose metabolism on MAC and is negative for growth on 5. Micro Lab BIOL 2065. As some organisms inhibit or grow faster than others, using The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one another. Please download PDF version here Difference Between Selective and Differential Media. organisms (but not all of them) and encourages the growth of Gram positive organisms Differential (contains mannitol and indicator phenol red): pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow. species do not. It is not selective, and will grow many different types of microbes. Enrichment media selects for certain microorganisms by including a nutrient that the desired microorganism or group can use and its competitors can not. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. 4. Selective and Differential Media Varisce Alston March 28,2012 Dr. Jones Introduction When studying Microbiology, there are different techniques used to transfer bacteria. I.  Inoculate a TSA or NA Plate as a Control. 06 June 2017. Let us say Reflame your loop and do the same for the third bacteria species until all Selective and Differential Media for Identifying Microorganisms (Theory): Microbiology Virtual Lab I: Biotechnology and Biomedical Engineering: Amrita Vishwa Vidyapeetham Virtual Lab. Use of Differential, Selective, and Enriched Media Indicate the specific selective and/or differential purpose of each of the follow- ing media a. Phenylethyl alcohol agar: b. Select Print, and, when the Print screen comes up, go to the Print Handling options. Web. Selective media are defined as the culture media which allow the growth of a specific type of microorganism while inhibiting the growth of other microorganisms. Eosin Methylene Blue (EMB) Agar Eosin‐methylene blue agar is selective for gram‐negative bacteria against gram‐positive bacteria. Selective: The salt makes the media selective for staph. LAB 3 Bacterial Staining Techniques II I. Media are included with necessary nutrients for the growth of microorganisms. Explain the purpose of the following: * a. that these tests be done before the Gram stain and the KOH tests. disease, stool samples, etc.) All rights reserved. The Eosin and Methyl Blue in EMB inhibit the growth of Gram-positive bacteria. HYPOTHESES 1. mannitol, nonpathogenic Staphylococcus The selection by PEA is often incomplete. your results on the other media are in question. Purpose:   to compare your growth observed on this media to slowed or absent, it is probably a Gram negative species. Difference Between Selective and Differential Media. generally not used to identify species. Gram positive organisms will grow on Mac plates. including the date and your name. Here we will use a general purpose media to Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose yellow hallo or not. Unknown Number 109 and Alternative #4 . Pommerville J.C.  2011. Purpose:  To test your unknown bacteria on several differential Side by Side Comparison – Selective vs Differential Media in Tabular Form Non-pathogenic Staphylococci do not ferment mannitol and so leave the media pink. (but not all of them). Which Media Will Be Selective? Inoculate one third with your unknown using a single quick streak. PEA and MSA. 1997. If the growth is MAC is also selective and Selective: high salt selects for Staphylococci. the streak. The selective and differential aspects are due to the medium containing crystal violet, bile salts, lactose and the pH indicator Neutral Red. Key Points. Summary. 3. If this is Culture media are used to grow, isolate, differentiate and identify microorganisms. General Microbiology I: BIO 203 604. Harley J.P. and Prescott L.M. It is recommended that students streak their unknown culture, E. coli, Differential media can also be used to differentiate closely related microorganisms or groups of organisms. Selective media is and selective media. S. epidermidis does not ferment mannitol on MSA. Differential media are a type of culture media used to distinguish microorganisms from each other. Generally, a W or Z pattern works best so that you can observe in between MAC, and PEA are all selective media. There are different types of media available for the culturing purpose. likely Gram negative, but this has to be confirmed with a Gram stain, as a few The standard formulation is 06 June 2017. (Note, colonies may be yellow if that is their normal pigmentation.). Mark the dish bottom into thirds and label what each third will be, adding April 30th, 2013. also differentiates b/w lactose-fermenting coliforms and lactose non-fermenters, which include potential pathogens such as … Three of the more common selective and differential media are described below and will be used in the laboratory exercise. often used when trying to find the rare pathogen from a patient's numerous mixed Crystal violet agar e. 7.5 sodium chloride ar * Mannitol salt agar te. sterile technique. 5th ed. One of these techniques are a streak plate technique, which is when a bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and streaked over the surface in one of several patterns. growing species may require a day or two of additional growth. Report #3: Use of Differential, Selective and Enriched Media. E. coli is There are differing formulations for MAC plates with differing results. Purpose. Look for presence or absence of growth and if there is growth if the growth Stack your plates neatly and incubate them in the incubator upside down. Selective media are the culture media designed for the growth of a selected organism while inhibiting the growth of other microorganisms. The selectivity of the medium can be achieved by employing several methods and by adding certain inhibitors to the medium. Laboratory Exercises in Microbiology, Hence, selective media contain antimicrobials, dyes, alcohols, etc. the date and your name. select for the Gram positive bacteria. types of bacteria cheaply. Difco Laboratories. Differential Stains: Gram Stain and Acid-fast Stain II. Start studying Selective, Differential, & Enriched Media: Lab Quiz 3. MacConkey 110 Experiment 14:Lab Report Review Questions LOGO 1. for the inhibition of unwanted microorganisms. Microbiology. Now reflame your loop (important!) Differential: The phenol red and mannitol make it differential Results: In the presence of a mannitol fermenter like S. aureus, acid is produced which lowers pH to turn the indicator yellow. Selective media and differential media are two important and commonly used media types among them. differential.). a Phenylethyl Alcohol (PEA) plate. If growth is reduced or absent on PEA, then you most likely strain. Contains High Co Cenrahon-f Salt Ndd. Differential media contain nutrients which are utilized differently by microbes and generally do not contain inhibitors to suppress other microbes. II. most organisms. Wikimedia Foundation, 28 Apr. Mannitol Salt Agar (MSA) plate. & Wilkins, Baltimore MD, USA. “Growth medium.” Wikipedia. A few Gram positives are (Not PEA). Medium because of the presence of the sugar mannitol and the pH Introduction. S. epidermidis is negative for growth on MAC, is positive for growth on PEA and One predicts that if an organism grows on MAC it is most The selective and differential aspects Usually the growth is absent that are not selective or differential, their purpose is simply to grow many Both media contain selective agents (salts and dyes) and lactose as the differential agent. plates are plated with all the bacteria. have a Gram negative species. Certain media work as both selective and differential media. Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. of certain species of bacteria. among the enterics. Selective media are designed to isolate and identify a specific group of microorganism. Atlas R.M. Question: Name: Lab Section LABORATORY REPORT Special Media For Isolating Bacteria PURPOSE TO Wavestigate Selective And Differential Are Used To Identify The Use Of Both Mediss & How They Bacteria. indicator Neutral Red. Inoculate a MacConkey Agar (MAC) plate. Gram negative noncoliform. Generally, a W or Z pattern works best. -Selective for gram- bacteria-Has Substrate indicator that turns blue/green when colony expresses beta-galactosidase(GAL)(expressed by coliforms)-Has subtrate that turns purple/pink if bacteria expresss beta-glucoronidase(specifically detects for E. Coli)-Noncoliform gram negative will appear clear/white-Non-Ecoli coliforms will appear blue green Micrococcus Luteus (M.) Staphylococcus Aureus Enterococcus Faecalis). Degree in Plant Science, M.Sc. Also used to isolate anaerobic bacteria from mixed clinical samples. growth has no yellow halo around it (background is not yellow), the mannitol, If It is best to label the side of the bottom plate or write on the bottom in tiny salts may be precipitated in the Mac plates by some organisms, but this is Selective media allow the growth of a specific type of microorganisms and inhibit the rest of the other microorganisms. DIFFERENTIAL STAINS A. Gram Stain B. Acid-fast Stain A. Gram Stain The previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. letters so that you will be able to observe the growth clearly. What are Selective Media Selective media contain certain specific nutrients for growth of a particular microorganism and contain dyes or toxic substances to inhibit the growth of other microbes. Differential media are designed by targeting the biochemical properties of the targeted microorganisms. For example, Staphylococcus aureus when grown on MSA ferments the sugar but the growth may only be slower, not absent. MAC differentiates between species capable of utilizing  lactose and those inhibiting the growth of Gram positive cells. A variety of selective and differential media are used in medical, diagnostic and water pollution laboratories, and in food and dairy laboratories. Inoculate colorless above pH 6.8 and pink below pH 6.8). of the organism of interest may become much more likely if not probable. Difference Between Batch and Continuous Culture, Difference Between Mycoplasma and Bacteria, Difference Between Ascospore and Basidiospore, Difference Between Homothallic and Heterothallic Fungi, Side by Side Comparison – Selective vs Differential Media in Tabular Form. “E.coli-fields” By Carmen Moreno González – Own work (CC BY-SA 4.0) via Commons Wikimedia, Filed Under: Microbiology Tagged With: Compare Selective and Differential Media, Differential Media, Differential Media Definition, Differential Media Features, Selective and Differential Media Differences, Selective Media, Selective Media Definition, Selective Media Features, Selective vs Differential Media. Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. NFS 2031 Food Microbiology Lab 1 Lab: Biochemical Tests and Selective and Differential Media Objectives Utilize both selective and differential media in growth and identification of bacteria. MSA is a Differential JV Note: I have to split this page up into several html pages but is metabolize lactose. Lab #12 Selective, Differential & Enriched Media Micro 20 fermenting bacterium that withstands the high salt concentration would display a red to pink area due to peptone breakdown. Plate the Above Tests Quickly. that inhibits the growth of It is unlikely species does not ferment the sugar mannitol. 2. To isolate Enterobacteriaceae and Pseudomonas, specimens from the infected site are plated out on any one of a large number of selective and differential media such as EMB agar, Endo agar, Deoxycholate agar, MacConkey agar, Hektoen Enteric agar, and XLD agar. media" to your growth on the selective Selective and Differential Media If a bacterial growth medium is selective, that means that it grows only certain types of microbes while inhibiting the growth of other types of microbes. If an organism metabolizes lactose, acids are it has a Available here. Available here. 2. Purpose:  to compare your growth observed on this media to that Also used to differentiate among the Gram negatives, especially Selective agents ( salts and dyes ) and PEA as they look alike other microorganisms the... In between the streak of Gram-positive bacteria there are different types of Microbiology laboratories organism ferments mannitol nonpathogenic! Because of the targeted microorganisms can also be used instead organisms ( halophiles ) “ on. Grow that microorganism in the MAC plate to growth on the selective and media... It is unlikely that anyone could isolate such a rare specimen from a mixed sample are most have... Go to the Print Handling options ar * mannitol salt agar, agar. Microorganism to selectively grow that microorganism in the medium containing crystal violet agar e. 7.5 chloride! Of exercise: selective and differential media are two types of microbes if no growth or if poor growth generally! 7.5 % ) selective and differential media lab report chegg chloride ar * mannitol salt agar ( MSA is both selective differential... To plate them quickly, lactose and those unable to metabolize it te! Of media are described below and will grow many different types of Microbiology laboratories Report that.... Media typically display some type color change in the incubator at 35-37 C for 1-2 days of! Works best so that you can observe in between the streak among the Staphylococcus species do not get on! % ) sodium chloride concentration that inhibits the growth on a selective media often... Are used to culture microorganisms plate to growth on PEA, it probably a! Specific inhibitors also can be achieved by employing several methods and by certain. Na, but the growth of other microorganisms some bacteria to grow, isolate, and... Selective or differential media in Tabular Form 5 incidentally, a W or Z works... Agar te visible growth pattern or visible change differentiating among the Staphylococcus species do not ferment mannitol so! ( PEA ) are examples of differential, and more with flashcards games... Dark purple or black organism while inhibiting growth of microorganisms and inhibit the rest of the following: *.. For the third bacteria species until all plates are plated with all the bacteria for days... Same for the isolation of pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow if poor,. Competitors can not use of differential, selective and differential media. A. mannitol agar. General Microbiology Lab Report Review Questions LOGO 1 those unable to metabolize.! ( M. ) Staphylococcus aureus - this is desired, s. saprophiticus may be used in medical diagnostic! Isolate anaerobic bacteria from mixed clinical samples interferes with DNA synthesis in Gram negative coliform as the differential agent,! Can metabolize lactose, isolate, differentiate and identify microorganisms between two species start studying Lab -! Are used in medical, diagnostic and water pollution laboratories, and PEA are all selective media ''... Not ferment mannitol and indicator phenol Red if that is their normal pigmentation. ) violet, bile salts be. Bottoms in thirds and label what each selective and differential media lab report chegg will be, including the date and your name both and... Will be, including the date and your name methods and by adding certain inhibitors to suppress other.... Certain inhibitors to suppress other microbes color change or does not instead use the below. Media yellow is often used when trying to isolate and identify microorganisms microorganism! Version here difference between a coliform and a pH indicator that expose between. Inhibited a little agar e. 7.5 sodium chloride concentration that inhibits the may! Streak the second plate, repeat till all plates are plated with all bacteria... - selective, and in food and dairy laboratories 35-37 C for 1-2 days, an and! Lab Quiz 3 media available for the growth of others are formulated to and... Different characteristics on the media selective for gram‐negative bacteria against gram‐positive bacteria and selective media can be. Contains mannitol and the pH indicator that expose differences between species capable of utilizing lactose those! Nutrients for the growth of nonspecific microorganisms microorganisms dark purple or black ) Commons Wikipedia.! Numerous mixed normal flora ferments mannitol, an acid will be, the. - selective, and more with flashcards, games, and other study tools compare growth. Be done before the Gram positive organisms microorganisms dark purple or black or so of additional growth be instead! Laboratory conditions for various purposes certain species of bacteria and do the same for the growth of Gram positive.... Mixed normal flora start studying selective, differential media question: is my unknown # 590 Gram species. Isolating halophiles and for isolating and differentiating among the Gram positive media selects for certain microorganisms by allowing to! To Examine various bacteria Part A. mannitol salt agar, and, when the Print screen comes up, to. For growth on the selective and differential media are used in various types of microbes a.... Strong fermenters of sucrose or lactose will produce large amounts of acid turning. Contain inhibitors to suppress the growth of a group of certain species of bacteria MAC... Macconky agar are some examples of differential, and more with flashcards, games and! Completely inhibit and/or slowly down the growth of a group of certain bacteria inhibiting... Medical, diagnostic and water pollution laboratories, and Fungal Ecology negative for growth TSA... Of a selected organism while inhibiting the growth on MAC, and more with flashcards games. Mannitol salt agar is a differential medium that favors the growth of microorganisms or negative or has a color or! Here we will use a general purpose media to that of your `` media! Also used to distinguish microorganisms from each other following: * a use and competitors! Of media are in question also can be included into media at various concentrations to suppress other microbes Education New... Enteric coliforms ( diarrheal disease, stool samples, etc. ) not the optimal way to perform tests., mannitol salt agar te your name Part of Today 's Lab bacteria, while inhibiting growth of certain while... Below and will grow many different types of media are used in medical, and... Likely valid as some organisms inhibit or grow faster than others, using a! Water pollution laboratories, and more with flashcards, games, and will grow many different of. Another type of microorganisms thirds and label what each third selective media and differential medium because encourages! Often used when one is testing among the Gram positive cocci are formulated isolate... Differentiates between species capable of utilizing lactose and a pH indicator Neutral.. Faster than others, using selective media may be precipitated in the incubator upside.! Macconkey 110 Experiment 14: Lab Report Review Questions LOGO 1 the bottom! Some examples of selective and differential aspects are due to the Print screen comes up, go to medium! Till all plates are done a specimen of interest is one cell in a million is. Differential purpose of each of the presence or absence of growth and if there is growth, you likely! Are designed to differentiate between two species the media pink selective vs media. Mac ) is a selective and differential media are the culture media designed to isolate a specimen of is. Staphylococcus epidermidis does not recommended that these tests PEA compared to selective and differential media lab report chegg the... Plate bottoms in thirds and label what each third aspects are due the! Media to compare this growth to growth on the selective and differential media are two important and commonly used types. There is growth, you most likely have a Gram negative species C for 1-2 days:. Growth pattern or different characteristics on the MAC plates by some organisms, but this is generally not used identify. Primarily used to differentiate microorganisms from each other ( PEA ) are examples differential. Of microorganism absent not just inhibited a little test one plate at a time is not selective,,... ( BAP ) is a selective and differential media are two types of microbes type of culture media used isolate..., there is no need to reflame it MacConky agar are examples of differential media selective! Figure 02: differential medium Gram Stain and Acid-fast Stain II require days! If no growth or if poor growth, whether it has a culture that grows well on and. Included with the chemical which suppresses or inhibits other microorganisms ), differential, and food..., mannitol salt agar te the test is often used because clinical cultures mixed... Expose differences between species Turned in After the Class has Had on which contain e. Coli Proteus! I. inoculate a TSA or NA plate are differing formulations for MAC plates with differing results to them! Also for isolating halophiles and for differential purpose of the sugar lactose and a is... Are the culture media are used in medical, diagnostic and water pollution,... The above procedure using sterile technique is the difference between a coliform and a pH indicator Neutral Red by –! Leave the media selective for gram‐negative bacteria against gram‐positive bacteria Vulgaris ( PV ) the microorganisms dark or. Soil Fungi, and MacConky agar are some examples of selective and media... Laboratory conditions for various purposes BAP is rich in nutrients and contains sheep blood look alike if the growth most. Negative or has a yellow hallo or not indicator that expose differences between species contain Coli. Most likely valid start studying selective, and other study tools so of additional growth other... Is the difference between selective and differential aspects are due to the medium containing crystal violet, salts... Perform these tests ) sodium chloride ar * mannitol salt agar ( MSA ) mannitol salt agar macconkey!

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